Mazia D, Brewer P A & Alfert M. The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biol. Bull. 104:56-67, 1953
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چکیده
Mercuric bromphenol blue, which has been used to visualize protein spots on filter paper, was studied with respect to its suitability as a quantitative cytochemical stain for tissue sections. The stain permits sharp visual differentiation of structural details and it follows the Beer-Lambert laws and can be used for microphotometry. It reacts with various sites in proteins, to the extent of about one dye binding group per ten amino acid residues. [The SCI indicates that this paper has been cited over 495 times since 1961.]
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Fischer, F. G. & Dorfel, H. (1953). Biochem. Z. 324, 544. Fiske, C. H. & Subbarow, Y. (1925). J. biol. Chem. 66, 375. Frick, G. (1954a). Biochim. biophys. Acta, 13, 41. Frick, G. (1954b). Biochim. biophys. Acta, 13, 374. Grassmann, W. & Deffner, G. (1953). Hoppe-Seyl. Z. 293, 89. Hyde, B. B. (1955). Biol. Bull., Woodc Hole, 109, 347. Jesaitis, M. A. & Goebel, W. F. (1955). J. exp. Med. 102, 733...
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The search for suitable staining methods for use in electron microscopy has led to many and diverse attempts to increase the opacity to electrons of different structures in tissue sections. The most obvious approach has been the attempt to bind heavy metals to specific sites, either to increase the general contrast or to identify the chemical nature of the structures so stained. Other than the ...
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Head, M. J. & Rook, J. A. F. (1957). Proc. Nutr. SOC. 16, 2 5 . McCance, R. A. & Widdowson, E. M. (1939). Biochen. J . 33, 523. McCance, R. A. & Widdowson, E. M. (1942-3). J. Physiol. 101, 350. McDonald, P. (1958). Proc. Brit. SOC. Anim. Prod. p. 31. Middleton, G. & Stuckey, R. E. (1954). Analyst, 79, 138. Powell, F. J. N. (1953). J . clin. Path. 6 , 286. Sjollema, B. (1931). Tierernahrung, 3, ...
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3Scott, G. H., Proc. Soc. Exptl. Biol. and Med., 45, 30-31 (1940). 4Allg6n, L. G., Acta Physiol. Scand., 22; Suppl., 76, 140 pp. (1950). 6 Gulick, A., Bot. Rev., 7, 433-457 (1941). 6 Milovidov, P. F., Protoplasma Monographien, 20, Erster Tell (1949). 7Poulson, D. F., and V. T. Bowen, Expil. Cell Research, Suppi., 2, 161-180 (1952). 8 Stubbe, H., and Doring, H., Z. indukt. Abstamm. u. Vererb. Le...
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Peters, J. P. & Van Slyke, D. D. (1931). Quantitative Clinical Chemistry, vol. 2. London: Bailliere, Tindall and Cox. Pfaltz, M. H. & Baudisch, 0. (1923). J. Amer. chem. Soc. 45, 2972. Rigg, T., Stein, G. & Weiss, J. (1952). Proc. Roy. Soc. A, 211, 375. Scholes, G., Stein, G. & Weiss, J. (1949). Nature, Lond., 164, 709. Scholes, G. & Weiss, J. (1950). Nature, Lond., 166, 640. Scholes, G. & Weis...
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